Report on Assembly Standards

EBP Assembly Standards
Version 7.0 | January 2026

The Earth BioGenome Project assembly standards define recommended minimum quality targets for reference genomes contributing to EBP goals. These standards are updated regularly to reflect advances in sequencing, assembly, curation, and data submission practices.

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At-a-Glance Standards

 

Table of Contents

  1. What Standard Applies to My Species?

  2. Core Assembly Metrics

  3. Additional Quality Requirements

  4. INSDC Submission Requirements

  5. Diploid and Polyploid Assemblies

  6. Tools for Measuring Assembly Quality

  7. References and Appendices

 

1. What Standard Applies to My Species?

 
 

Figure 1: Overview of the INSDC BioProject, assembly and raw data submission structure. Add haplotype assembly BioProjects and data as required in case of polyploid eukaryotes. Equally, add as required for multiple prokaryotic assemblies, e.g. Wolbachias. Eukaryotic cobionts will require their own tolid and BioProject. Umbrella projects can be linked and stacked to reflect their relationships. If several individuals of the same species are assembled and released by the same project, their data BioProjects can be added to the species umbrella BioProject.